Scientists Discover How Proteins form Crystals that Tile a Microbe`s Shell

Many microbes wear beautifully patterned crystalline shells. which protect them from a harsh world and can even help them reel in food. Studies at the Department of Energy`s SLAC National Accelerator Laboratory and Stanford University have revealed this food-reeling process and shown how shells assemble themselves from protein building blocks.

Now the same team has zoomed in on the very first step in microbial shell-building: nucleation. where squiggly proteins crystallize into sturdy building blocks. much like rock candy crystallizes around a string dipped into sugar syrup.

The results. published today in the Proceedings of the National Academy of Sciences. could shed light on how the shells help microbes interact with other organisms and with their environments. and also help scientists design self-assembling nanostructures for various tasks.

Jonathan Herrmann. a graduate student in Professor Soichi Wakatsuki`s group at SLAC and Stanford. collaborated with the structural molecular biology team at SLAC`s Stanford Synchrotron Radiation Lightsource (SSRL) on the study. They scattered a powerful beam of X-rays off protein molecules that were floating in a solution to see how the atomic structures of the molecules changed as they nucleated into crystals. Meanwhile. other researchers made a series of cryogenic electron microscope (cryo-EM) images at various points in the nucleation process to show what happened over time.

They found out that crystal formation takes place in two steps: One end of the protein molecule nucleates into crystal while the other end. called the N-terminus. continues to wiggle around. Then the N-terminus joins in. and the crystallization is complete. Far from being a laggard. the N-terminus actually speeds up the initial nucleation step – although exactly how it does this is still unknown. the researchers said — and this helps explain why microbial shells can form so quickly and efficiently.

 

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